Translesion synthesis (TLS) relies on a series of specialized DNA polymerases able to insert a base either correctly or incorrectly opposite a lesion on a DNA template strand during replication or post-repair synthesis. To measure the correct or mutagenic outcome of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) bypass by TLS DNA polymerases, a capillary electrophoresis (CE) method with fluorescent label ...

Parentage assessment in the potato (Solanum tuberosum L.), based on a reliable characterization of DNA varieties, can be a useful tool in validating pedigree information as well as for assigning potential parents to orphan varieties. The approach suggested in this study was to use the genetic profiles obtained on 577 cultivars using a set of 17 polymorphic simple sequence repeats (SSR) markers in ...

The aim of this study was to underscore that seed treatment by the fog of ozonated water constitutes a promising alternative tool, in terms of health and environmental gains, regarding traditional ozone treatment. In order to obtain a clear vision of this performance, the technology was implemented on an industrial scale (malting industry). Under an exposition of barley seeds to 9.8 ppm of dissolved ...

Résumé des auteurs : Monitoring the change in genetic diversity over time and space in crop species is essential to facilitating further improvement. As the world’s most important tuber crop for human consumption, and an ideal candidate to help address global food security, the cultivated potato deserves in-depth study in this regard. In this overview, some aspects of spatiotemporal diversity ...

Abstract : "Improved knowledge of the diversity within and among local animal populations is increasingly necessary for their sustainable management. Accordingly, this study assessed the genetic diversity and structure of the indigenous goat population of Benin. Nine hundred and fifty-four goats were sampled across the three vegetation zones of Benin [i.e., Guineo-Congolese zone (GCZ), Guineo-Sudanian ...