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Measurement of translesion synthesis by fluorescent capillary electrophoresis : 7,8-Dihydro-8-oxodeoxyguanosine bypass modulation by natural products
24 mai 2013, Nachtergael, Amandine; Charles, Catherine; SPANOGHE, Martin; GADENNE, Martine; Belayew, Alexandra; Duez, Pierre,
- HE Condorcet
,
- Article scientifique
Article scientifique
Translesion synthesis (TLS) relies on a series of specialized DNA polymerases able to insert a base either correctly or incorrectly opposite a lesion on a DNA template strand during replication or post-repair synthesis. To measure the correct or mutagenic outcome of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) bypass by TLS DNA polymerases, a capillary electrophoresis (CE) method with fluorescent label ...
Use of self-organizing map to analyze images of fungi colonies grown from Triticum aestivum seeds disinfected by ozone treatment
16 octobre 2012, MARIQUE, Thierry; ALLARD, Olivier; SPANOGHE, Martin,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
We submitted to ozone treatment Triticum aestivum (common wheat) seeds severely contaminated by fungi. Fungi colonies developed when seeds were placed over malt agar medium in Petri dishes; Fusarium sp. and Alternaria sp. were identified. However, conventional colonies counting did not allow a clear assessment of the effect of ozone disinfection. We thus used self-organizing maps (SOMs) to perform ...