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Use of self-organizing map to analyze images of fungi colonies grown from Triticum aestivum seeds disinfected by ozone treatment
16 octobre 2012, MARIQUE, Thierry; ALLARD, Olivier; SPANOGHE, Martin,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
We submitted to ozone treatment Triticum aestivum (common wheat) seeds severely contaminated by fungi. Fungi colonies developed when seeds were placed over malt agar medium in Petri dishes; Fusarium sp. and Alternaria sp. were identified. However, conventional colonies counting did not allow a clear assessment of the effect of ozone disinfection. We thus used self-organizing maps (SOMs) to perform ...
Measurement of translesion synthesis by fluorescent capillary electrophoresis : 7,8-Dihydro-8-oxodeoxyguanosine bypass modulation by natural products
24 mai 2013, Nachtergael, Amandine; Charles, Catherine; SPANOGHE, Martin; GADENNE, Martine; Belayew, Alexandra; Duez, Pierre,
- HE Condorcet
,
- Article scientifique
Article scientifique
Translesion synthesis (TLS) relies on a series of specialized DNA polymerases able to insert a base either correctly or incorrectly opposite a lesion on a DNA template strand during replication or post-repair synthesis. To measure the correct or mutagenic outcome of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) bypass by TLS DNA polymerases, a capillary electrophoresis (CE) method with fluorescent label ...
Investigation and development of potato parentage analysis methods using multiplexed SSR fingerprinting
25 décembre 2014, SPANOGHE, Martin; MARIQUE, Thierry; RIVIÈRE, John; LANTERBECQ, Déborah; GADENNE, Martine,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
Parentage assessment in the potato (Solanum tuberosum L.), based on a reliable characterization of DNA varieties, can be a useful tool in validating pedigree information as well as for assigning potential parents to orphan varieties. The approach suggested in this study was to use the genetic profiles obtained on 577 cultivars using a set of 17 polymorphic simple sequence repeats (SSR) markers in ...
Industrial-scale Malting Barley (Hordeum vulgare L.) Seed Disinfection by Fog of Ozonated Water Application
05 novembre 2015, SPANOGHE, Martin; ALLARD, Olivier; DELVOYE, Sébastien; MARIQUE, Thierry; Van Koninckxloo, Michel,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
The aim of this study was to underscore that seed treatment by the fog of ozonated water constitutes a promising alternative tool, in terms of health and environmental gains, regarding traditional ozone treatment. In order to obtain a clear vision of this performance, the technology was implemented on an industrial scale (malting industry). Under an exposition of barley seeds to 9.8 ppm of dissolved ...
Development and application of a quantitative real-time PCR assay for rapid detection of the multifaceted yeast Kazachstania servazzii in food.
avril 2017, SPANOGHE, Martin; Godoy Jara, Mario; RIVIÈRE, John; LANTERBECQ, Déborah; GADENNE, Martine; MARIQUE, Thierry,
- CARAH
,
- Article scientifique
Article scientifique
The beneficial contributions of Kazachstania servazzii are well-established in various food processes. This yeast also contributes in the spoilage of finished packaged food due to abundant gas production. In particular, an occurrence of K. servazzii was recently positively correlated with the formation of severe package swelling of some prepared fresh pizzas. To circumscribe this concern, a quantitative ...