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Measurement of translesion synthesis by fluorescent capillary electrophoresis : 7,8-Dihydro-8-oxodeoxyguanosine bypass modulation by natural products
24 mai 2013, HE CondorcetArticle scientifiqueTranslesion synthesis (TLS) relies on a series of specialized DNA polymerases able to insert a base either correctly or incorrectly opposite a lesion on a DNA template strand during replication or post-repair synthesis. To measure the correct or mutagenic outcome of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) bypass by TLS DNA polymerases, a capillary electrophoresis (CE) method with fluorescent label ...