| dc.rights.license | CC0 | en_US |
| dc.contributor.author | COURTOIS, Justine | |
| dc.contributor.author | Bertholet, Catherine | |
| dc.contributor.author | TOLLENAERE, Stéphanie | |
| dc.contributor.author | Van der Brempt, Xavier | |
| dc.contributor.author | Cavalier, Etienne | |
| dc.date.accessioned | 2020-05-19T09:41:20Z | |
| dc.date.available | 2020-05-19T09:41:20Z | |
| dc.date.issued | 2020 | |
| dc.identifier.issn | 10.1016/j.jpba.2019.112907 | en_US |
| dc.identifier.uri | https://luck.synhera.be/handle/123456789/261 | |
| dc.identifier.doi | 10.1016/j.jpba.2019.112907 | en_US |
| dc.description.abstract | 178(2020), 13 p. | en_US |
| dc.description.abstractfr | L'allergie au blé est relativement courante et les manifestations cliniques associées dépendent des allergènes moléculaires impliqués ainsi que du mode d'exposition. Différents symptômes ont été décrits: anaphylaxie induite par l'exercice liée au blé (WDEIA), dermatite atopique (AD) et rhinite pollinique (PR). Les méthodes de diagnostic traditionnelles ne permettent pas une identification moléculaire précise des allergènes essentiels à l'évaluation des risques et au choix du traitement le plus adapté. | en_US |
| dc.description.abstracten | Wheat allergy is relatively common and the associated clinical manifestations depend on the involved molecular allergens as well as on the way of exposure. Different symptoms have been described: wheat-dependent exercise-induced anaphylaxis (WDEIA), atopic dermatitis (AD) and pollen rhinitis (PR). Traditional diagnostic methods do not allow accurate molecular identification of the allergens that are essential for risk assessment and for the choice of the most adapted treatment. | en_US |
| dc.description.tableofcontents | 1. Background
2. Material and methods
2.1. Preparation of standardized wheat extracts
2.2. Patient selection
2.3. 2D Western blot
2.4. Marker peptide selection
2.5. Tryptic digestion from SDS-PAGE gel
2.6. Protein identification by UHPLC-MS/MS
3. Results
3.1. 2D electrophoresis
3.2. Analysis of patients’ sera using 2D WB
3.2.1. EIA group
3.2.2. AD group
3.2.3. PR group
3.3. Identification of proteins of interest by UHPLC-MS/MS
4. Discussion
5. Conclusions
Authors’ contribution
Declaration of competing interests
Availability of data and materials
Ethics approval and consent to participate
Funding
References | en_US |
| dc.language.iso | EN | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | en_US |
| dc.relation.isreferencedby | 0731-7085 | en_US |
| dc.rights.uri | ? | en_US |
| dc.subject | Wheat allergy | en_US |
| dc.subject | Wheat allergens | en_US |
| dc.subject | Mass spectrometry | en_US |
| dc.subject | Western blot | en_US |
| dc.subject.fr | Allergie au blé | en_US |
| dc.subject.fr | Allergènes du blé | en_US |
| dc.subject.fr | Spectrométrie de masse | en_US |
| dc.subject.fr | Western blot | en_US |
| dc.title | Detection of wheat allergens using 2D Western blot and mass spectrometry | en_US |
| dc.title.fr | Détection d'allergènes de blé par Western blot 2D et spectrométrie de masse | en_US |
| dc.type | Article scientifique | en_US |
| synhera.classification | Sciences de la santé humaine | en_US |
| synhera.institution | HELMo | en_US |
| synhera.institution | CRIG | en_US |
| synhera.otherinstitution | ULiège | en_US |
| synhera.otherinstitution | CHU Liège | en_US |
| synhera.otherinstitution | Allergopöle, Clinique Saint-Luc, Bouge | en_US |
| synhera.otherinstitution | CER Group Marloie | en_US |
| synhera.stakeholders.fund | ? | en_US |
| synhera.cost.total | 1 | en_US |
| synhera.cost.apc | 1 | en_US |
| synhera.cost.comp | 1 | en_US |
| synhera.cost.acccomp | 1 | en_US |
| dc.description.version | Oui | en_US |
| dc.rights.holder | Elsevier | en_US |
