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Pedigree Validation and Reconstruction Applied in the Cultivated Potato Using 35 Microsatellites Markers
05 juin 2023,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
Abstract ; "Knowledge of the parental origin of crop species is a prerequisite for formulating efective strategies for genetic conservation, management, and utilisation of genotypes in breeding programmes and genetic studies. In the case of the potato, although pedigree information is well documented, the possible lack of accurate pedigree data for certain cultivars constitutes a loss of opportunity ...
Development of a standardized multiplex SSR kit for genotyping both goats and sheep
03 novembre 2021,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
"In this technical note, we report the development of a reliable standardized protocol based on the multiplex SSRPCR approach for genotyping the two small ruminant species, goats and sheep. The method is based on PCR conditions optimization allowing 12 informative microsatellites (SSR) markers to be co-amplified in two multiplex sets of 6 SSR markers, followed by a co-migration into one run onto a ...
Genetic Diversity Trends in the Cultivated Potato: A Spatiotemporal Overview
15 avril 2022,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
Résumé des auteurs : Monitoring the change in genetic diversity over time and space in crop species is essential to facilitating further improvement. As the world’s most important tuber crop for human consumption, and an ideal candidate to help address global food security, the cultivated potato deserves in-depth study in this regard. In this overview, some aspects of spatiotemporal diversity ...
Genetic diversity and spatial structure of indigenous sheep population of Benin revealed by microsatellite markers
12 août 2022,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
"This study investigated the genetic variability existing within the indigenous sheep population of Benin. Hair samples from 681 unrelated sheep collected across the 10 phytogeographic zones of Benin were genotyped using a set of 12 microsatellite markers"
Extrait de l'abstract
Development and application of a quantitative real-time PCR assay for rapid detection of the multifaceted yeast Kazachstania servazzii in food.
avril 2017,
- CARAH
,
- Article scientifique
Article scientifique
The beneficial contributions of Kazachstania servazzii are well-established in various food processes. This yeast also contributes in the spoilage of finished packaged food due to abundant gas production. In particular, an occurrence of K. servazzii was recently positively correlated with the formation of severe package swelling of some prepared fresh pizzas. To circumscribe this concern, a quantitative ...
Effects of Chemopreventive Natural Compounds on the Accuracy of 8-oxo-7,8-dihydro-2′-deoxyguanosine Translesion Synthesis
08 juillet 2021,
- HE Condorcet
,
- Article scientifique
Article scientifique
Translesion synthesis is a DNA damage tolerance mechanism that relies on a series of specialized DNA polymerases able to bypass a lesion on a DNA template strand during replication or post-repair synthesis. Specialized translesion synthesis DNA polymerases pursue replication by inserting a base opposite to this lesion, correctly or incorrectly depending on the lesion nature, involved DNA polymerase(s), ...
Industrial-scale Malting Barley (Hordeum vulgare L.) Seed Disinfection by Fog of Ozonated Water Application
05 novembre 2015,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
The aim of this study was to underscore that seed treatment by the fog of ozonated water constitutes a promising alternative tool, in terms of health and environmental gains, regarding traditional ozone treatment. In order to obtain a clear vision of this performance, the technology was implemented on an industrial scale (malting industry). Under an exposition of barley seeds to 9.8 ppm of dissolved ...
Investigation and development of potato parentage analysis methods using multiplexed SSR fingerprinting
25 décembre 2014,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
Parentage assessment in the potato (Solanum tuberosum L.), based on a reliable characterization of DNA varieties, can be a useful tool in validating pedigree information as well as for assigning potential parents to orphan varieties. The approach suggested in this study was to use the genetic profiles obtained on 577 cultivars using a set of 17 polymorphic simple sequence repeats (SSR) markers in ...
Measurement of translesion synthesis by fluorescent capillary electrophoresis : 7,8-Dihydro-8-oxodeoxyguanosine bypass modulation by natural products
24 mai 2013,
- HE Condorcet
,
- Article scientifique
Article scientifique
Translesion synthesis (TLS) relies on a series of specialized DNA polymerases able to insert a base either correctly or incorrectly opposite a lesion on a DNA template strand during replication or post-repair synthesis. To measure the correct or mutagenic outcome of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) bypass by TLS DNA polymerases, a capillary electrophoresis (CE) method with fluorescent label ...
Use of self-organizing map to analyze images of fungi colonies grown from Triticum aestivum seeds disinfected by ozone treatment
16 octobre 2012,
- HE Condorcet
- CARAH
,
- Article scientifique
Article scientifique
We submitted to ozone treatment Triticum aestivum (common wheat) seeds severely contaminated by fungi. Fungi colonies developed when seeds were placed over malt agar medium in Petri dishes; Fusarium sp. and Alternaria sp. were identified. However, conventional colonies counting did not allow a clear assessment of the effect of ozone disinfection. We thus used self-organizing maps (SOMs) to perform ...