Pedigree Validation and Reconstruction Applied in the Cultivated Potato Using 35 Microsatellites Markers

Abstract

Abstract ; "Knowledge of the parental origin of crop species is a prerequisite for formulating efective strategies for genetic conservation, management, and utilisation of genotypes in breeding programmes and genetic studies. In the case of the potato, although pedigree information is well documented, the possible lack of accurate pedigree data for certain cultivars constitutes a loss of opportunity to exploit the selection gain, the precise targeting of a market niche, or other breeding objectives. In this study, the genetic profles of 1249 varieties were examined using a set of 35 polymorphic simple sequence repeats (SSR) markers with the available pedigree information to achieve pedigree validation. The maximum likelihood (LOD) was used to validate pedigree information of potato accessions for which the genetic data of at least one parent was available in the dataset. Our results showed that among the accessions for which both parents were genotyped, 81.7% presented a validated pedigree, while 18.3% presented a pedigree confict (for at least one parent). Among the accessions for which only one parent was available in the dataset, 91.3% presented a validated pedigree, while 8.7% presented a pedigree confict. Then, pedigree reconstruction was investigated to determine the most likely parent candidates for varieties whose pedigree is either questionable or lacking information."