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Date
2011-06-23Auteur
Desoignies, Nicolas
Legrève, Anne
Metadata
Afficher la notice complèteIn Vitro Dual Culture of Polymyxa betae in Agrobacterium rhizogenes Transformed Sugar Beet Hairy Roots in Liquid Media
Résumé
"Polymyxa betae is a soil-borne protist and an obligate parasite of sugar beet that transmits the beet necrotic yellow vein virus. Sugar beet hairy roots, transformed by Agrobacterium rhizogenes, were inoculated with surface-sterilized root fragments infected by P. betae. After 10 wk in a liquid medium, typical structures of P. betae were observed in this in vitro system. This first in vitro culture of P. betae in liquid medium will contribute to a better understanding of this protist's biology through providing a way to conserve and produce purified isolates of the protist."