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Effects of Chemopreventive Natural Compounds on the Accuracy of 8-oxo-7,8-dihydro-2′-deoxyguanosine Translesion Synthesis
08 juillet 2021, Nachtergael, Amandine; LANTERBECQ, Déborah; SPANOGHE, Martin; Belayew, Alexandra; Duez, Pierre, HE CondorcetArticle scientifiqueTranslesion synthesis is a DNA damage tolerance mechanism that relies on a series of specialized DNA polymerases able to bypass a lesion on a DNA template strand during replication or post-repair synthesis. Specialized translesion synthesis DNA polymerases pursue replication by inserting a base opposite to this lesion, correctly or incorrectly depending on the lesion nature, involved DNA polymerase(s), ... -
Measurement of translesion synthesis by fluorescent capillary electrophoresis : 7,8-Dihydro-8-oxodeoxyguanosine bypass modulation by natural products
24 mai 2013, Nachtergael, Amandine; Charles, Catherine; SPANOGHE, Martin; GADENNE, Martine; Belayew, Alexandra; Duez, Pierre, HE CondorcetArticle scientifiqueTranslesion synthesis (TLS) relies on a series of specialized DNA polymerases able to insert a base either correctly or incorrectly opposite a lesion on a DNA template strand during replication or post-repair synthesis. To measure the correct or mutagenic outcome of 7,8-dihydro-8-oxodeoxyguanosine (8-oxodG) bypass by TLS DNA polymerases, a capillary electrophoresis (CE) method with fluorescent label ...